Format

Send to

Choose Destination
Bioconjug Chem. 1990 May-Jun;1(3):222-6.

Simplified method for conjugating macrocyclic bifunctional chelating agents to antibodies via 2-iminothiolane.

Author information

1
Chemistry Department, University of California, Davis 95616.

Abstract

A one-step method for conjugating macrocyclic chelators to antibodies using the protein modification reagent 2-iminothiolane controls aggregation, maintains immunoreactivity, and produces consistent chelate/antibody ratios. Conjugation conditions have been investigated with the macrocyclic chelates 6-[p-(bromoacetamido)benzyl]-1,4,8,11-tetraazacyclotetradecane-N,N ',N",N"'-tetraacetic acid and 2-[p-(bromoacetamido)benzyl]-1,4,7,10-tetraazacyclododecane-N,N',N ",N"'-tetraacetic acid, with three different monoclonal antibodies. The bifunctional chelating agents are prepared by bromoacetylation of their amine precursors using a two-phase H2O/CHCl3 system, which improves product purity.

PMID:
2096914
DOI:
10.1021/bc00003a007
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center