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Methods Mol Biol. 2011;670:231-43. doi: 10.1007/978-1-60761-744-0_16.

Real-time visualization of axonal transport in neurons.

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1
Department of Chemistry, Stanford University, Stanford, CA, USA.

Abstract

The normal function of neurons depends on the integrity of microtubule-dependent transport of cellular materials and organelles to/from their cell bodies or axon terminus. In this chapter, we describe the design and implementation of a fluorescence imaging method to visualize axonal transport in neurons directly. We combine a pseudo total internal reflection microscopy, quantum dot fluorescence labeling, microfluidic neuronal culture chamber, and single molecule detection methods to achieve a high spatial and temporal resolution in tracking nerve growth factor transport in dorsal root ganglia neurons.

PMID:
20967594
DOI:
10.1007/978-1-60761-744-0_16
[Indexed for MEDLINE]
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