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Methods Mol Biol. 2011;670:33-44. doi: 10.1007/978-1-60761-744-0_3.

Isolation of low-n amyloid β-protein oligomers from cultured cells, CSF, and brain.

Author information

1
Center for Neurologic Diseases, Brigham & Women's Hospital, Harvard Medical School, Boston, MA, USA.

Abstract

Recent data suggest that soluble, non-fibrillar assemblies of the amyloid β-protein (Aβ) may mediate the synaptic deficits that characterize the early stages of Alzheimer's disease. Consequently, much effort has been expended in isolating and studying a variety of different Aβ assemblies. Here, we describe the use of immunoprecipitation/western blotting and size exclusion chromatography/western blotting to characterize Aβ present in conditioned medium from cultured cells, human cerebrospinal fluid, and human cortex extracted with aqueous buffer, detergent, and formic acid.

PMID:
20967581
DOI:
10.1007/978-1-60761-744-0_3
[Indexed for MEDLINE]

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