Format

Send to

Choose Destination
FEBS Lett. 2010 Nov 19;584(22):4606-10. doi: 10.1016/j.febslet.2010.10.023. Epub 2010 Oct 20.

Methyl-beta-cyclodextrin induces mitochondrial cholesterol depletion and alters the mitochondrial structure and bioenergetics.

Author information

1
Department of Biochemistry, J. Sniadecki Academy of Physical Education and Sport, K. Gorskiego 1, 80-336 Gdansk, Poland. wiech@awf.gda.pl

Abstract

There is growing evidence of mitochondrial membrane raft-like microdomains that are involved in the apoptotic pathway. The aim of this study was to investigate the effect of methyl-beta-cyclodextrin (MβCD), being a well-known lipid microdomain disrupting agent and cholesterol chelator, on the structure and bioenergetics of rat liver mitochondria (RLM). We observed that MβCD decreases the function of RLM, induces changes in the mitochondrial configuration state and decreases the calcium chloride-induced swelling. These data suggest that disruption of mitochondrial raft-like microdomains by cholesterol efflux on one hand impairs mitochondrial bioenergetics, but on the other hand it protects the mitochondria from swelling.

PMID:
20965172
DOI:
10.1016/j.febslet.2010.10.023
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center