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J Biochem Toxicol. 1990 Winter;5(4):269-72.

Spectroscopic quantitation of cytochrome P-450 in human lung microsomes.

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Department of Pharmacology, University of Illinois College of Medicine, Chicago 60612.


The cytochrome P-450 content of human lung microsomes was measured by difference spectroscopy of the carbon monoxide-complexed hemoprotein. These measurements were only possible after the microsome preparation had been subjected to centrifugation over a discontinuous sucrose gradient, to remove an opaque black contaminant. The specific concentration of total cytochrome P-450 in human lung microsomes is essentially identical to that of microsomes prepared under identical conditions from untreated baboon lungs, but is only 0.7% of the specific content found in lung microsomes from untreated rabbits. These measurements correspond well to the observed metabolic capacities of the various microsome samples.

[Indexed for MEDLINE]

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