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Biochimie. 1990 Dec;72(12):881-4.

Improved method for measuring the catalytic activity of tryptophan synthase alpha subunit in cell extracts.

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1
Department of Biochemistry, University of Southern California, School of Medicine 90033.

Abstract

An improved method has been developed for measuring the catalytic activity of tryptophan synthase alpha-subunit in cell extracts using the indole-3-glycerol phosphate (InGP)----tryptophan reaction. The method involves the chemical and enzymatic synthesis of the substrate InGP immediately before use and avoids the preparation of salt-free hydroxylamine. The method is more convenient, safer and more reliable than the traditional method employing the InGP----indole reaction.

PMID:
2095923
[Indexed for MEDLINE]

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