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Appl Biochem Biotechnol. 2011 Apr;163(8):954-64. doi: 10.1007/s12010-010-9099-5. Epub 2010 Oct 15.

Vectors for glucose-dependent protein expression in Saccharomyces cerevisiae.

Author information

1
Institut für Genetik, Technische Universität Dresden, 01062 Dresden, Germany. simone.thierfelder@tu-dresden.de

Abstract

Based on the p426 series of expression vectors developed by Mumberg et al. (Gene 156, 119-122, 1995), we have generated a set of plasmids that allow the glucose-dependent expression of target genes in the yeast, Saccharomyces cerevisiae. The ADH1 promoter in plasmid p426-ADH1 was replaced by the 1-kb 5'-region from either of the following genes: HXK1, YGR243, HXT4 and HXT7. Expression mediated by the respective 5'-regions was monitored with EGFP, yEGFP3-CLN2pest and TurboGFP as marker genes. Fluorescence is induced 2.7-fold using the HXK1, 2.3-fold using the YGR243-, 5-fold using the HXT7- and 12.6-fold using the HXT4 5'-regions upon depletion of glucose to a concentration of <0.5 g/l.

PMID:
20949330
DOI:
10.1007/s12010-010-9099-5
[Indexed for MEDLINE]

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