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Nat Rev Microbiol. 2010 Nov;8(11):791-801. doi: 10.1038/nrmicro2423.

Organotypic 3D cell culture models: using the rotating wall vessel to study host-pathogen interactions.

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Center for Infectious Diseases and Vaccinology, Arizona State University, Tempe, AZ 85287, USA.


Appropriately simulating the three-dimensional (3D) environment in which tissues normally develop and function is crucial for engineering in vitro models that can be used for the meaningful dissection of host-pathogen interactions. This Review highlights how the rotating wall vessel bioreactor has been used to establish 3D hierarchical models that range in complexity from a single cell type to multicellular co-culture models that recapitulate the 3D architecture of tissues observed in vivo. The application of these models to the study of infectious diseases is discussed.

[Indexed for MEDLINE]

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