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EMBO Rep. 2010 Nov;11(11):883-9. doi: 10.1038/embor.2010.152. Epub 2010 Oct 15.

Protein phosphatase 1γ is responsible for dephosphorylation of histone H3 at Thr 11 after DNA damage.

Author information

1
Department of Cell Biology, Graduate School of Medical Sciences, Nagoya City University, Nagoya, Japan.

Abstract

The DNA-damage-induced transcriptional suppression of cell cycle regulatory genes correlates with a reduction in histone H3-Thr 11 phosphorylation (H3-pThr 11) on their promoters that is partly mediated by the dissociation of Chk1 from chromatin. In this study, we identify protein phosphatase 1γ (PP1γ) as a phosphatase responsible for DNA-damage-induced H3-pThr 11 dephosphorylation. PP1γ is activated after DNA damage, which is mainly mediated by a reduction in Cdk-dependent phosphorylation of PP1γ at Thr 311. The depletion of PP1γ sensitizes HCT116 cells to DNA damage. Our results suggest that the ataxia telangiectasia, mutated and Rad3-related-Chk1 axis regulates H3-pThr 11 dephosphorylation on DNA damage, at least in part by the activation of PP1γ through Chk1-dependent inhibition of Cdks.

PMID:
20948546
PMCID:
PMC2966955
DOI:
10.1038/embor.2010.152
[Indexed for MEDLINE]
Free PMC Article

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