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Science. 2010 Oct 15;330(6002):390-393. doi: 10.1126/science.1194598.

Salmonella pathogenesis and processing of secreted effectors by caspase-3.

Author information

1
Department of Pediatric Gastroenterology and Nutrition, Harvard Medical School and Massachusetts General Hospital, Boston, MA 02129, USA.
2
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA.
3
Institute of Infection, Immunity & Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, G12 8QQ, UK.
4
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907.
#
Contributed equally

Abstract

The enteric pathogen Salmonella enterica serovar Typhimurium causes food poisoning resulting in gastroenteritis. The S. Typhimurium effector Salmonella invasion protein A (SipA) promotes gastroenteritis by functional motifs that trigger either mechanisms of inflammation or bacterial entry. During infection of intestinal epithelial cells, SipA was found to be responsible for the early activation of caspase-3, an enzyme that is required for SipA cleavage at a specific recognition motif that divided the protein into its two functional domains and activated SipA in a manner necessary for pathogenicity. Other caspase-3 cleavage sites identified in S. Typhimurium appeared to be restricted to secreted effector proteins, which indicates that this may be a general strategy used by this pathogen for processing of its secreted effectors.

PMID:
20947770
PMCID:
PMC4085780
DOI:
10.1126/science.1194598
[Indexed for MEDLINE]
Free PMC Article

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