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Methods Enzymol. 2010;470:429-46. doi: 10.1016/S0076-6879(10)70017-3. Epub 2010 Mar 1.

Imaging single mRNA molecules in yeast.

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1
Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

Abstract

Yeast cells in an isogenic population do not all display the same phenotypes. To study such variation within a population of cells, we need to perform measurements on each individual cell instead of measurements that average out the behavior of a cell over the entire population. Here, we provide the basic concepts and a step-by-step protocol for a recently developed technique enabling one such measurement: fluorescence in situ hybridization that renders single mRNA molecule visible in individual fixed cells.

PMID:
20946820
DOI:
10.1016/S0076-6879(10)70017-3
[Indexed for MEDLINE]
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