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Methods Enzymol. 2010;470:51-75. doi: 10.1016/S0076-6879(10)70003-3. Epub 2010 Mar 1.

Genome-wide approaches to monitor pre-mRNA splicing.

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1
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York, USA.

Abstract

Pre-mRNA processing is an essential control-point in the gene expression pathway of eukaryotic organisms. The budding yeast Saccharomyces cerevisiae offers a powerful opportunity to examine the regulation of this pathway. In this chapter, we will describe methods that have been developed in our lab and others to examine pre-mRNA splicing from a genome-wide perspective in yeast. Our goal is to provide all of the necessary information--from microarray design to experimental setup to data analysis--to facilitate the widespread use of this technology.

PMID:
20946806
DOI:
10.1016/S0076-6879(10)70003-3
[Indexed for MEDLINE]
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