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Opt Express. 2010 Aug 30;18(18):18875-85. doi: 10.1364/OE.18.018875.

Achieving increased resolution and more pixels with Superresolution Optical Fluctuation Imaging (SOFI).

Author information

1
Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, California, USA. t.dertinger@chem.ucla.edu

Abstract

Superresolution Optical Fluctuation Imaging (SOFI) as initially demonstrated allows for a resolution enhancement in imaging by a factor of square-root of two. Here, we demonstrate how to increase the resolution of SOFI images by re-weighting the Optical Transfer Function (OTF). Furthermore, we demonstrate how cross-cumulants can be exploited to obtain a fair approximation of the underlying Point-Spread Function. We show a two-fold increase of resolution (over the diffraction limit) of near-infrared quantum dot labeled tubulin-network of 3T3 fibroblasts.

PMID:
20940780
PMCID:
PMC3072111
DOI:
10.1364/OE.18.018875
[Indexed for MEDLINE]
Free PMC Article

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