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J Struct Biol. 2011 Apr;174(1):234-8. doi: 10.1016/j.jsb.2010.10.002. Epub 2010 Oct 16.

Graphene: Substrate preparation and introduction.

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Center for Cellular Imaging and Nano Analytics, Biozentrum, University of Basel, Basel, Switzerland.


This technical note describes the transfer of continuous, single-layer, pristine graphene to standard Quantifoil TEM grids. We compare the transmission properties of pristine graphene substrates to those of graphene oxide and thin amorphous carbon substrates. Positively stained DNA imaged across amorphous carbon is typically indiscernible and requires metal shadowing for sufficient contrast. However, in a practical illustration of the new substrates properties, positively stained DNA is imaged across pristine graphene in striking contrast without the need of metal shadowing. We go onto discuss technical considerations and the potential applications of pristine graphene substrates as well as their ongoing development.

[Indexed for MEDLINE]

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