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Chin J Dent Res. 2010;13(1):45-9.

Involvement of Notch signalling pathway in senescence of human dental pulp cells.

Author information

1
Department of Cariology and Endodontology, Peking University School and Hospital of Stonmatology, Beijing, PR China.

Abstract

OBJECTIVE:

To evaluate whether the Notch signalling pathway is involved in the senescence of human dental pulp cells.

METHODS:

Human dental pulp cells were isolated and cultured. The Notch signalling pathway was blocked by adding DAPT (N-[N-(3,5-difluorophenacetyl-L-alanyl)]-S-phenylglycine t-butyl ester, γ-secretase inhibitor, 5 μmol/L) into the culture medium. Cell proliferation was evaluated by MTT assay. Cell senescence was evaluated through expression of senescenceassociated β-galactosidase (SA-β-Gal) using β-galactosidase staining.

RESULTS:

The proliferation of the human dental pulp cells decreased dramatically after treatment with DAPT, compared with the control. SA-β-Gal positive staining cells were seen in the DAPT group, but not in the control group.

CONCLUSION:

The Notch signalling pathway is involved in the senescence of human dental pulp cells.

PMID:
20936191
[Indexed for MEDLINE]
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