Synthetic riboswitches that induce gene expression in diverse bacterial species

Appl Environ Microbiol. 2010 Dec;76(23):7881-4. doi: 10.1128/AEM.01537-10. Epub 2010 Oct 8.

Abstract

We developed a series of ligand-inducible riboswitches that control gene expression in diverse species of Gram-negative and Gram-positive bacteria, including human pathogens that have few or no previously reported inducible expression systems. We anticipate that these riboswitches will be useful tools for genetic studies in a wide range of bacteria.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Bacteria / genetics*
  • Gene Expression*
  • Genetic Engineering / methods*
  • Genetics, Microbial / methods*
  • Riboswitch / drug effects*

Substances

  • Riboswitch