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Appl Environ Microbiol. 2010 Dec;76(23):7741-53. doi: 10.1128/AEM.00819-10. Epub 2010 Oct 8.

Repression of antibiotic production and sporulation in Streptomyces coelicolor by overexpression of a TetR family transcriptional regulator.

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1
Group Métabolisme Energétique des Streptomyces, Institut de Génétique et Microbiologie, UMR CNRS 8621, Université Paris Sud 11, 91405 Orsay Cedex, France. xudelin@live.com

Abstract

The overexpression of a regulatory gene of the TetR family (SCO3201) originating either from Streptomyces lividans or from Streptomyces coelicolor was shown to strongly repress antibiotic production (calcium-dependent antibiotic [CDA], undecylprodigiosin [RED], and actinorhodin [ACT]) of S. coelicolor and of the ppk mutant strain of S. lividans. Curiously, the overexpression of this gene also had a strong inhibitory effect on the sporulation process of S. coelicolor but not on that of S. lividans. SCO3201 was shown to negatively regulate its own transcription, and its DNA binding motif was found to overlap its -35 promoter sequence. The interruption of this gene in S. lividans or S. coelicolor did not lead to any obvious phenotypes, indicating that when overexpressed SCO3201 likely controls the expression of target genes of other TetR regulators involved in the regulation of the metabolic and morphological differentiation process in S. coelicolor. The direct and functional interaction of SCO3201 with the promoter region of scbA, a gene under the positive control of the TetR-like regulator, ScbR, was indeed demonstrated by in vitro as well as in vivo approaches.

PMID:
20935121
PMCID:
PMC2988594
DOI:
10.1128/AEM.00819-10
[Indexed for MEDLINE]
Free PMC Article
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