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Curr Opin Struct Biol. 2010 Oct;20(5):606-14. doi: 10.1016/j.sbi.2010.09.002. Epub 2010 Oct 9.

Hard X-ray fluorescence tomography--an emerging tool for structural visualization.

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1
Australian Synchrotron, 800 Blackburn Road, Clayton, Victoria 3168, Australia. martin.dejonge@synchrotron.org.au

Abstract

Hard X-ray fluorescence microscopy is well-suited to in-situ investigations of trace metal distributions within whole, unstained, biological tissue, with sub-parts-per-million detection achievable in whole cells. The high penetration of X-rays indicates the use of X-ray fluorescence tomography for structural visualization, and recent measurements have realised sub-500-nm tomography on a 10-μm cell. Limitations of present approaches impact the duration of an experiment and imaging fidelity. Developments in X-ray resolution, detector speed, cryogenic environments, and the incorporation of auxiliary signals are being pursued within the synchrotron community. Several complementary approaches to X-ray fluorescence tomography will be routinely available to the biologist in the near future. We discuss these approaches and review applications of biological relevance.

PMID:
20934872
DOI:
10.1016/j.sbi.2010.09.002
[Indexed for MEDLINE]
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