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Methods Mol Biol. 2011;678:147-77. doi: 10.1007/978-1-60761-682-5_12.

Transposon insertional mutagenesis in rice.

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1
CSIRO Plant Industry, Canberra, ACT, Australia.

Abstract

Insertion mutants offer one of the direct ways to relate a gene to its function by employing forward or reverse genetics approaches. Both T-DNA and transposon insertional mutants are being produced in several crops, including rice, the first cereal with its complete genome sequenced. Transposons have several advantages over T-DNA including the ability to produce multiple independent insertion lines from individual starter lines, as well as producing revertants by remobilization. With our new gene constructs, and a two-component transposon iAc/Ds mutagenesis protocol, we have improved both gene trapping and screening efficiencies in rice.

PMID:
20931379
DOI:
10.1007/978-1-60761-682-5_12
[Indexed for MEDLINE]
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