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Hua Xi Yi Ke Da Xue Xue Bao. 1990 Sep;21(3):271-3.

[Human sex identification in dried bloodstains by analysis of amplified DNA sequences].

[Article in Chinese]


This paper describes a technique of DNA amplification in vitro and its application on sex identification of human dried bloodstains. Dried bloodstains (8 males and 8 females) were prepared on filter paper, and then DNA was extracted. Target DNA sequences (Y-3.4kb repeat and Alu-repeat) were amplified by polymerase chain reaction (PCR) with two pairs of primers (Y1.1, Y1.2 and Alu9.1, Alu9.2). Amplification products were obtained in 8 male's bloodstains with the primers Y1.1 and Y1.2, but not in 8 female's bloodstains; and in 4 male's and 4 female's bloodstains with Alu9.1 and Alu9.2.

[Indexed for MEDLINE]

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