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Chem Commun (Camb). 2010 Nov 21;46(43):8195-7. doi: 10.1039/c0cc02864a. Epub 2010 Oct 6.

Identification of epigenetic DNA modifications with a protein nanopore.

Author information

1
Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford, UK OX1 3TA.

Abstract

Two DNA bases, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (hmC), marks of epigenetic modification, are recognized in immobilized DNA strands and distinguished from G, A, T and C by nanopore current recording. Therefore, if further aspects of nanopore sequencing can be addressed, the approach will provide a means to locate epigenetic modifications in unamplified genomic DNA.

PMID:
20927439
PMCID:
PMC3147113
DOI:
10.1039/c0cc02864a
[Indexed for MEDLINE]
Free PMC Article

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