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Ann Clin Biochem. 2010 Nov;47(Pt 6):524-8. doi: 10.1258/acb.2010.010033. Epub 2010 Oct 6.

Analytical characteristics of the Roche highly sensitive troponin T assay and its application to a cardio-healthy population.

Author information

1
ACT Pathology, The Canberra Hospital, Woden, ACT 2605, Australia. gus.koerbin@act.gov.au

Abstract

BACKGROUND:

It is desirable that current assays for cardiac troponin (cTn) are able to meet the recommended criterion that the diagnosis and risk assessment of patients present with symptoms of myocardial infarction requires a rise and fall in cTn with at least one point above the 99th percentile of a reference population. We have evaluated the analytical characteristics of the new highly sensitive troponin T (hs-TnT) assay to see if it meets this criterion and applied it to a carefully defined, cardio-healthy Australian reference population.

METHODS:

An imprecision profile was determined for the Roche hs-TnT assay (Roche Diagnostics, Sydney, Australia) using multiple samples analysed on nine separate occasions. The distribution of troponin T was determined using 104 samples from a cardio-healthy population.

RESULTS:

The new hs-TnT assay meets the specifications of a coefficient of variation of 10% at the 99th percentile of our cardio-healthy reference population. Of the 104 samples analysed 44 showed troponin T concentrations above the manufacturer's quoted limit of detection. Age and gender differences in the median and 99th percentile troponin T concentration were observed.

CONCLUSIONS:

The new hs-TnT assay shows improved precision and sensitivity at very low troponin concentration. We have shown that a significant number of individuals in this cardio-healthy population had detectable circulating troponin concentration. With many apparently healthy people having detectable troponin, clinical judgement will become more important in interpreting troponin results.

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PMID:
20926463
DOI:
10.1258/acb.2010.010033
[Indexed for MEDLINE]

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