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AIDS Res Hum Retroviruses. 2010 Oct;26(10):1155-61. doi: 10.1089/aid.2010.0041. Epub 2010 Oct 6.

Inhibition of X4-tropic HIV type 1 replication by knockdown of the cellular protein LEREPO4.

Author information

1
Department of Hematology/Oncology and Infectious Diseases, J.W. Goethe-University Hospital, Frankfurt/Main, Germany.

Abstract

Human immunodeficiency virus 1 (HIV-1) and host cell factors show important mutual interactions. We found that HIV-1 infection induced expression of a likely ortholog of mouse immediate early response erythropoietin 4 (LEREPO4) in vitro. When LEREPO4 expression was suppressed by siRNA in P4-CCR5 cells, HIV-1 replication showed significantly reduced HIV-1 transcript and p24 protein levels as measured by quantitative PCR and ELISA, respectively. The LEREPO4 knockdown also had an inhibitory effect on HIV-1-LTR-driven reporter plasmid expression of β-galactosidase. Furthermore, the inhibitory effect of LEREPO4 silencing on HIV-1 replication was confirmed in Jurkat T cells. The up-regulation of LEREPO4 by HIV-1 and the inhibition of HIV-1 replication mediated by knockdown of LEREPO4 may point to an important functional role of LEREPO4 as a novel HIV-1 dependency factor.

PMID:
20925576
DOI:
10.1089/aid.2010.0041
[Indexed for MEDLINE]

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