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Arch Pathol Lab Med. 2010 Oct;134(10):1534-40. doi: 10.1043/2009-0581-OA.1.

Antihuman leukocyte antigen–specific antibody strength determined by complement-dependent or solid-phase assays can predict positive donor-specific crossmatches.

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1
Departments of Pathology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA.

Abstract

CONTEXT:

The association of circulating donor-specific antibody (DSA) strength with crossmatch results is of potential interest to predict allograft outcome.

OBJECTIVES:

To systematically investigate the aforementioned association and to attempt to define a cutoff value for DSA strength that can predict a positive crossmatch result.

DESIGN:

We analyzed DSA strength and crossmatch results from the 2006 to 2008 proficiency testing samples of the American Society of Histocompatibility and Immunogenetics (n  =  50). We further validated our findings in candidates for potential kidney transplant (n  =  19).

RESULTS:

Proficiency test samples with positive antihuman globulin T-cell crossmatch results had significantly higher DSA strength, as assessed by Luminex (Austin, Texas) mean fluorescent intensity (MFI; MFI [SD], 7860 [4770]), compared with samples with negative crossmatch results (MFI [SD], 2900 [1820]; P  =  .001). Similarly, higher Luminex values were observed in samples from candidates for transplant with positive antihuman globulin T-cell crossmatch results (MFI [SD], 7910 [2370] versus 2840 [1960]; P < .001). The MFI value of 6540 had 61% and 75% sensitivity and 92% and 94% specificity for predicting positive antihuman globulin T-cell crossmatches in proficiency test samples and in candidates for transplant, respectively.

CONCLUSIONS:

The DSA strength correlates well with crossmatch results. An MFI of 6540 predicted a positive antihuman globulin T-cell crossmatch.

PMID:
20923311
DOI:
10.1043/2009-0581-OA.1
[Indexed for MEDLINE]
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