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J Proteome Res. 2010 Nov 5;9(11):6082-9. doi: 10.1021/pr100924s. Epub 2010 Oct 15.

One-step sample concentration, purification, and albumin depletion method for urinary proteomics.

Author information

1
Department of Urology, Harvard Medical School, Boston, Massachusetts 02115, USA.

Abstract

Workflows in urinary proteomics studies are often complex and require many steps to enrich, purify, deplete, and separate the complex mixture. Many of these methods are laborious, are time-consuming, and have the potential for error. Although individual steps of these methods have been previously studied, their downstream compatibilities with fractionation technologies such as off-gel electrophoresis have not been investigated. We developed a one-step sample preparation workflow that simultaneously (i) concentrates proteins, (ii) purifies by removing salts and other low molecular weight compounds, and (iii) depletes (albumin) from urine samples. This simple and robust workflow can be multiplexed and is compatible with a diverse range of downstream multidimensional separation technologies. Additionally, because of its high reproducibility and flexibility in processing samples with different volumes and concentrations, it has the potential to be used for standardization of urinary proteomics studies, as well as for studying other body fluids of similar complexity.

PMID:
20923230
PMCID:
PMC2974758
DOI:
10.1021/pr100924s
[Indexed for MEDLINE]
Free PMC Article

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