Sizing nanomatter in biological fluids by fluorescence single particle tracking

Nano Lett. 2010 Nov 10;10(11):4435-42. doi: 10.1021/nl103264u. Epub 2010 Oct 5.

Abstract

Accurate sizing of nanoparticles in biological media is important for drug delivery and biomedical imaging applications since size directly influences the nanoparticle processing and nanotoxicity in vivo. Using fluorescence single particle tracking we have succeeded for the first time in following the aggregation of drug delivery nanoparticles in real time in undiluted whole blood. We demonstrate that, by using a suitable surface functionalization, nanoparticle aggregation in the blood circulation is prevented to a large extent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Chemical Analysis / methods*
  • Humans
  • Image Interpretation, Computer-Assisted / methods*
  • Microscopy, Fluorescence / methods*
  • Nanostructures / analysis*
  • Nanostructures / ultrastructure*
  • Particle Size*