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J Biochem Biophys Methods. 1990 Nov-Dec;21(4):277-84.

Electrostatic assay of phospholipase A activity: an application of the second harmonic method of monitoring membrane boundary potentials.

Author information

1
Frumkin Institute of Electrochemistry U.S.S.R. Academy of Sciences, Moscow.

Abstract

To evaluate phospholipase A activity a new assay is suggested. This assay is based on the recording of boundary potential changes of the planar bilayer lipid membrane during enzymatic hydrolysis of lipids. To register these changes, a second harmonic method is used. Sensitivity of the assay is about 0.0002 units/ml regardless of the impurities that may be present in the samples. One analysis takes about 5 min.

PMID:
2089069
DOI:
10.1016/0165-022x(90)90002-t
[Indexed for MEDLINE]

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