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Methods Enzymol. 2010;482:369-80. doi: 10.1016/S0076-6879(10)82015-4.

Correcting for the ewald sphere in high-resolution single-particle reconstructions.

Author information

1
Department of Applied Physics, California Institute of Technology, Pasadena, California, USA.

Abstract

To avoid the challenges of crystallization and the size limitations of NMR, it has long been hoped that single-particle cryo-electron microscopy (cryo-EM) would eventually yield atomically interpretable reconstructions. For the most favorable class of specimens (large icosahedral viruses), one of the key obstacles is curvature of the Ewald sphere, which leads to a breakdown of the Projection Theorem used by conventional three-dimensional (3D) reconstruction programs. Here, we review the basic problem and our implementation of the "paraboloid" reconstruction method, which overcomes the limitation by averaging information from images recorded from different points of view.

PMID:
20888969
PMCID:
PMC3716286
DOI:
10.1016/S0076-6879(10)82015-4
[Indexed for MEDLINE]
Free PMC Article

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