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J Microbiol Methods. 2010 Dec;83(3):302-6. doi: 10.1016/j.mimet.2010.09.018. Epub 2010 Oct 1.

Rapid homogeneous PCR assay for the detection of Chlamydia trachomatis in urine samples.

Author information

1
Department of Biotechnology, University of Turku, Tykistökatu 6A, 20520 Turku, Finland. artule@utu.fi

Abstract

Chlamydia trachomatis infection is the most common bacterial sexually transmitted disease and a major public health problem worldwide. Fast and sensitive point-of-care diagnostics including non-invasive sample collection would be of value for the prevention of C. trachomatis transmission. The aim of this study was to develop a fast, reliable, non-invasive and easy-to-use homogenous PCR assay for the detection of C. trachomatis. Bacteria were concentrated from urine by a simple and fast centrifugation-based urine pretreatment method. Novel automated GenomEra technology was utilized for the rapid closed-tube PCR including time-resolved fluorometric detection of the target using lanthanide chelate labeled probes. We have developed a rapid C. trachomatis assay which provides qualitative results in 1 h with diagnostic sensitivity and specificity of 98.7% and 97.3%, respectively. The novel assay can be performed with minimal laboratory expertise and without sophisticated DNA-extraction devices and has performance comparable to current gold standard assays.

PMID:
20888867
DOI:
10.1016/j.mimet.2010.09.018
[Indexed for MEDLINE]

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