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Toxicon. 2010 Dec;56(8):1497-505. doi: 10.1016/j.toxicon.2010.09.008. Epub 2010 Oct 1.

Apoptogenic peptides from Tityus discrepans scorpion venom acting against the SKBR3 breast cancer cell line.

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1
Laboratory on Cellular Neuropharmacology, Biophysics and Biochemistry Center, Instituto Venezolano de Investigaciones Cientificas (IVIC) Caracas, Venezuela. gina.dsuze@gmail.com

Abstract

Two novel peptides named neopladine 1 and neopladine 2 were purified from Tityus discrepans scorpion venom and found to be active on human breast carcinoma SKBR3 cells. Mass spectrometry molecular masses of neopladine 1 and 2 were 29918 and 30388 Da, respectively. Their N-terminal sequences were determined by Edman degradation. The peptides induced apoptosis of SKBR3 cells but had a negligible effect on non-malignant MA104 monkey kidney cells. Neopladine 1 and 2 induced 6.3 and 4.1% of SKBR3 apoptosis, respectively, in 5 h of exposure; the effect was larger with more prolonged exposures. Inmunohistochemistry showed that neopladines bind to SKBR3 cell surface inducing FasL and BcL-2 expression.

PMID:
20888852
DOI:
10.1016/j.toxicon.2010.09.008
[Indexed for MEDLINE]
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