Functional annotation of heart enriched mitochondrial genes GBAS and CHCHD10 through guilt by association

Biochem Biophys Res Commun. 2010 Nov 12;402(2):203-8. doi: 10.1016/j.bbrc.2010.09.109. Epub 2010 Oct 1.

Abstract

Despite the mitochondria ubiquitous nature many of their components display divergences in their expression profile across different tissues. Using the bioinformatics-approach of guilt by association (GBA) we exploited these variations to predict the function of two so far poorly annotated genes: Coiled-coil-helix-coiled-coil-helix domain containing 10 (CHCHD10) and glioblastoma amplified sequence (GBAS). We predicted both genes to be involved in oxidative phosphorylation. Through in vitro experiments using gene-knockdown we could indeed confirm this and furthermore we asserted CHCHD10 to play a role in complex IV activity.

MeSH terms

  • Adenosine Triphosphate / biosynthesis*
  • Computational Biology / methods
  • Electron Transport Complex IV / metabolism*
  • Gene Knockdown Techniques
  • Genes, Mitochondrial / physiology*
  • HeLa Cells
  • Heart / physiology*
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins / genetics
  • Membrane Proteins / physiology*
  • Mitochondrial Proteins / genetics
  • Mitochondrial Proteins / physiology*
  • Oxidative Phosphorylation*
  • Phosphoproteins / genetics
  • Phosphoproteins / physiology*

Substances

  • CHCHD10 protein, human
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Mitochondrial Proteins
  • NIPSNAP2 protein, human
  • Phosphoproteins
  • Adenosine Triphosphate
  • Electron Transport Complex IV