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Methods Enzymol. 2010;483:121-42. doi: 10.1016/S0076-6879(10)83006-X.

Cryoelectron microscopy applications in the study of tubulin structure, microtubule architecture, dynamics and assemblies, and interaction of microtubules with motors.

Author information

1
Life Sciences Division, Donner Laboratory, Lawrence Berkeley National Laboratory, Berkeley, California, USA.

Abstract

Cryo-EM is ideally suited for the study of cytoskeleton polymers and their interaction with cellular partners. Our understanding of microtubule (MT) structure and interactions has benefited tremendously from the application of different EM techniques, from the use of electron crystallography to determine the first high-resolution structure of tubulin, to electron tomographic reconstructions of unique MT-based organelles; from molecular details governing the regulated interaction of MTs with kinesin motors, to an atomic description of how antimitotic agents bind to tubulin and affect MT stability. In this chapter, we review these structural findings with an emphasis on how cryo-EM enables our studies.

PMID:
20888472
PMCID:
PMC4165512
DOI:
10.1016/S0076-6879(10)83006-X
[Indexed for MEDLINE]
Free PMC Article

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