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Methods Enzymol. 2010;481:195-230. doi: 10.1016/S0076-6879(10)81009-2.

Site-specific biomolecule labeling with gold clusters.

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1
Colorado State University, Department of Chemistry, Fort Collins, Colorado, USA.

Abstract

Site-specific labeling of biomolecules in vitro with gold clusters can enhance the information content of electron cryomicroscopy experiments. This chapter provides a practical overview of well-established techniques for forming biomolecule/gold cluster conjugates. Three bioconjugation chemistries are covered: linker-mediated bioconjugation, direct gold-biomolecule bonding, and coordination-mediated bonding of nickel(II) nitrilotriacetic acid (NTA)-derivatized gold clusters to polyhistidine (His)-tagged proteins.

PMID:
20887859
PMCID:
PMC3568671
DOI:
10.1016/S0076-6879(10)81009-2
[Indexed for MEDLINE]
Free PMC Article
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