Tin oxide nanoparticles-polymer modified single-use sensors for electrochemical monitoring of label-free DNA hybridization

Talanta. 2010 Oct 15;82(5):1680-6. doi: 10.1016/j.talanta.2010.07.040. Epub 2010 Jul 24.

Abstract

In this study, SnO(2) nanoparticles (SNPs)-poly(vinylferrocenium) (PVF(+)) modified single-use graphite electrodes were developed for electrochemical monitoring of DNA hybridization. The surfaces of polymer modified and polymer-SNP modified pencil graphite electrodes (PGEs) were firstly characterized by using SEM analysis. The electrochemical behaviours of these electrodes were also investigated using the differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques. The polymer-SNP modified PGEs were then tested for the electrochemical sensing of DNA based on the changes at the guanine oxidation signals. Experimental parameters, such as; different modifications in DNA oligonucleotides, DNA probe concentrations were examined to obtain more sensitive and selective electrochemical signals for nucleic acid hybridization. After optimization studies, DNA hybridization was investigated in the case of complementary of hepatitis B virus (HBV) probe, mismatch (MM), and noncomplementary (NC) sequences.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Pair Mismatch
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods*
  • DNA Probes / chemistry*
  • Electrochemical Techniques / instrumentation
  • Electrochemical Techniques / methods*
  • Electrodes
  • Ferrous Compounds / chemistry*
  • Graphite
  • Oxidation-Reduction
  • Polyvinyls / chemistry*
  • Quantum Dots*
  • Tin Compounds / chemistry*

Substances

  • DNA Probes
  • Ferrous Compounds
  • Polyvinyls
  • Tin Compounds
  • polyvinylferrocenium
  • Graphite
  • stannic oxide