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Crit Rev Microbiol. 2011 Feb;37(1):15-24. doi: 10.3109/1040841X.2010.506177. Epub 2010 Sep 29.

Traditional mould analysis compared to a DNA-based method of mould analysis.

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United States Environmental Protection Agency, Cincinnati OH, USA.


Traditional environmental mould analysis is based on microscopic observations and counting of mould structures collected from the air on a sticky surface or culturing of moulds on growth media for identification and quantification. These approaches have significant limitations. A DNA-based method of mould analysis called mould specific quantitative PCR (MSQPCR) was created for more than 100 moulds. Based on a national sampling and analysis by MSQPCR of dust in US homes, a scale for comparing the mould burden in homes was created called the Environmental Relative Mouldiness Index (ERMI).

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