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Phys Rev Lett. 2010 Jun 4;104(22):225501. Epub 2010 Jun 2.

Cryptotomography: reconstructing 3D Fourier intensities from randomly oriented single-shot diffraction patterns.

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Laboratory of Atomic and Solid State Physics, Cornell High Energy Synchrotron Source (CHESS), Cornell University, Ithaca, New York 14853-2501, USA.

Erratum in

  • Phys Rev Lett. 2010 Jun 11;104(23):239902.


We reconstructed the 3D Fourier intensity distribution of monodisperse prolate nanoparticles using single-shot 2D coherent diffraction patterns collected at DESY's FLASH facility when a bright, coherent, ultrafast x-ray pulse intercepted individual particles of random, unmeasured orientations. This first experimental demonstration of cryptotomography extended the expansion-maximization-compression framework to accommodate unmeasured fluctuations in photon fluence and loss of data due to saturation or background scatter. This work is an important step towards realizing single-shot diffraction imaging of single biomolecules.

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