Format

Send to

Choose Destination
See comment in PubMed Commons below
Eur J Immunol. 2010 Nov;40(11):3226-34. doi: 10.1002/eji.201040349. Epub 2010 Sep 24.

Kinase suppressor of Ras 1 is required for full ERK activation in thymocytes but not for thymocyte selection.

Author information

1
Department of Pathology and Immunology, Washington University School of Medicine, Saint Louis, MO, USA.

Abstract

The scaffold protein kinase suppressor of Ras 1 (KSR1) is critical for efficient activation of ERK in a number of cell types. Consistent with this, we observed a defect in ERK activation in thymocytes that lack KSR1. Interestingly, we found that the defect was much greater after PMA stimulation than by CD3 activation. Since ERK activation is believed to be important for thymocyte development, we analyzed thymocyte selection in KSR1-deficient (KSR1(-/-) ) mice. We found that positive selection in two different TCR transgenic models, HY and AND, was normal. On the other hand, negative selection in the HY model was slightly impaired in KSR1(-/-) mice. However, a defect in negative selection was not apparent in the AND TCR model system or in an endogenous superantigen-mediated model of negative selection. These results suggest that, despite a requirement for KSR1 for full ERK activation in thymocytes, full and efficient ERK activation is not essential for the majority of thymocyte selection events.

PMID:
20865788
PMCID:
PMC2976810
DOI:
10.1002/eji.201040349
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley Icon for PubMed Central
    Loading ...
    Support Center