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Mol Cell. 2010 Sep 24;39(6):912-924. doi: 10.1016/j.molcel.2010.08.023.

HSP90 and its R2TP/Prefoldin-like cochaperone are involved in the cytoplasmic assembly of RNA polymerase II.

Boulon S#1, Pradet-Balade B#2,3,4, Verheggen C#2,3,4, Molle D#2,3,4, Boireau S2,3,4, Georgieva M2,3,4, Azzag K2,3,4, Robert MC2,3,4, Ahmad Y1, Neel H2,3, Lamond AI1, Bertrand E2,3,4.

Author information

1
Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee DD1 5EH, UK.
2
Institut de Génétique Moléculaire de Montpellier - UMR 5535 CNRS, Université Montpellier 2, 34293 Montpellier Cedex 5, France.
3
Université Montpellier 2, 34095 Montpellier Cedex 5, France.
4
Université Montpellier 1, 34060 Montpellier Cedex 2, France.
#
Contributed equally

Abstract

RNA polymerases are key multisubunit cellular enzymes. Microscopy studies indicated that RNA polymerase I assembles near its promoter. However, the mechanism by which RNA polymerase II is assembled from its 12 subunits remains unclear. We show here that RNA polymerase II subunits Rpb1 and Rpb3 accumulate in the cytoplasm when assembly is prevented and that nuclear import of Rpb1 requires the presence of all subunits. Using MS-based quantitative proteomics, we characterized assembly intermediates. These included a cytoplasmic complex containing subunits Rpb1 and Rpb8 associated with the HSP90 cochaperone hSpagh (RPAP3) and the R2TP/Prefoldin-like complex. Remarkably, HSP90 activity stabilized incompletely assembled Rpb1 in the cytoplasm. Our data indicate that RNA polymerase II is built in the cytoplasm and reveal quality-control mechanisms that link HSP90 to the nuclear import of fully assembled enzymes. hSpagh also bound the free RPA194 subunit of RNA polymerase I, suggesting a general role in assembling RNA polymerases.

PMID:
20864038
PMCID:
PMC4333224
DOI:
10.1016/j.molcel.2010.08.023
[Indexed for MEDLINE]
Free PMC Article

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