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J Virol. 2010 Dec;84(23):12454-7. doi: 10.1128/JVI.01792-10. Epub 2010 Sep 22.

Effect of time delay after necropsy on analysis of simian varicella-zoster virus expression in latently infected ganglia of rhesus macaques.

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1
Department of Neurology, University of Colorado School of Medicine, Aurora, Colorado 80045, USA. ravi.mahalingam@ucdenver.edu

Abstract

Studies of varicella-zoster virus gene expression during latency require the acquisition of human ganglia at autopsy. Concerns have been raised that the virus might reactivate immediately after death. Because features of varicella-zoster virus latency are similar in primate and human ganglia, we examined virus gene expression in tissues either processed immediately or kept at 4°C for 30 h before necropsy of two monkeys inoculated with simian varicella-zoster virus and euthanized 117 days later. Virus transcription and the detection of open reading frame (ORF) 63 protein in the cytoplasm of neurons were comparable. Thus, a 30-h delay after death did not affect varicella-zoster virus expression in latently infected ganglia.

PMID:
20861271
PMCID:
PMC2976390
DOI:
10.1128/JVI.01792-10
[Indexed for MEDLINE]
Free PMC Article

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