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Macromol Biosci. 2010 Nov 10;10(11):1355-64. doi: 10.1002/mabi.201000124.

Multifunctional hybrid three-dimensionally woven scaffolds for cartilage tissue engineering.

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1
Departments of Orthopaedic Surgery and Biomedical Engineering, Duke University Medical Center, 375 MSRB, Box 3093, Durham, NC 27710, USA.

Abstract

The successful replacement of large-scale cartilage defects or osteoarthritic lesions using tissue-engineering approaches will likely require composite biomaterial scaffolds that have biomimetic mechanical properties and can provide cell-instructive cues to control the growth and differentiation of embedded stem or progenitor cells. This study describes a novel method of constructing multifunctional scaffolds for cartilage tissue engineering that can provide both mechanical support and biological stimulation to seeded progenitor cells. 3-D woven PCL scaffolds were infiltrated with a slurry of homogenized CDM of porcine origin, seeded with human ASCs, and cultured for up to 42 d under standard growth conditions. These constructs were compared to scaffolds derived solely from CDM as well as 3-D woven PCL fabric without CDM. While all scaffolds promoted a chondrogenic phenotype of the ASCs, CDM scaffolds showed low compressive and shear moduli and contracted significantly during culture. Fiber-reinforced CDM scaffolds and 3-D woven PCL scaffolds maintained their mechanical properties throughout the culture period, while supporting the accumulation of a cartilaginous extracellular matrix. These findings show that fiber-reinforced hybrid scaffolds can be produced with biomimetic mechanical properties as well as the ability to promote ASC differentiation and chondrogenesis in vitro.

PMID:
20857388
PMCID:
PMC3214627
DOI:
10.1002/mabi.201000124
[Indexed for MEDLINE]
Free PMC Article

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