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Res Vet Sci. 2011 Aug;91(1):58-63. doi: 10.1016/j.rvsc.2010.08.007. Epub 2010 Sep 19.

Autoantibodies to recombinant canine proinsulin in canine diabetic patients.

Author information

1
Dept. of Pathology and Infectious Diseases, Royal Veterinary College, London, UK. lucy.davison@cimr.cam.ac.uk

Abstract

OBJECTIVE:

To determine whether dogs with spontaneously-occurring diabetes mellitus demonstrate serological reactivity to proinsulin.

SAMPLE POPULATION:

Serum samples were collected from 15 newly-diagnosed diabetic, 15 insulin-treated diabetic and 15 non-diabetic control dogs.

PROCEDURES:

Canine proinsulin was cloned into a prokaryotic expression vector to generate recombinant poly-histidine-tagged protein in Escherichia coli. A Western blotting assay was developed for detection of proinsulin autoantibodies in canine sera.

RESULTS:

Reactivity to canine proinsulin was detected in 3 of 15 control dogs, 8 of 15 newly-diagnosed diabetic dogs and 6 of 15 insulin-treated diabetic patients. Of these reactors, only 1 control dog, 1 newly-diagnosed diabetic dog and 3 insulin-treated diabetic dogs recognised porcine insulin by ELISA, suggesting that the remaining proinsulin reactors might have been recognising proinsulin-specific epitopes.

CONCLUSIONS AND CLINICAL RELEVANCE:

This study suggests that proinsulin autoantibodies are present in a proportion of diabetic dogs. Further work is required to refine the assay and clarify the significance of these autoantibodies.

PMID:
20855094
DOI:
10.1016/j.rvsc.2010.08.007
[Indexed for MEDLINE]

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