Format

Send to

Choose Destination
Biotechniques. 2010 Sep;49(3):659-61. doi: 10.2144/000113491.

Auto-induction medium containing glyphosate for high-level incorporation of unusual aromatic amino acids into proteins.

Author information

1
Biochemistry Department, University of Wisconsin-Madison, Madison, WI 53706, USA.

Abstract

We describe the use of an auto-induction medium containing N-(phosphono-methyl)glycine (glyphosate) as a means for high-level introduction of nonstandard aromatic amino acids into a protein. We illustrate this approach by preparing maltose binding protein (MBP) wherein all eight tryptophan residues have been replaced with 6-fluorotryptophan at an incorporation level of 99.3%. Such a high level of incorporation is important for spectroscopic investigations, in particular 19F NMR, because each species' differing amino acid sequence potentially yields a different peak pattern that complicates spectral analysis.

PMID:
20854268
PMCID:
PMC3023234
DOI:
10.2144/000113491
[Indexed for MEDLINE]
Free PMC Article

Publication type, MeSH terms, Substances, Grant support

Publication type

MeSH terms

Substances

Grant support

Supplemental Content

Full text links

Icon for Atypon Icon for PubMed Central
Loading ...
Support Center