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Appl Environ Microbiol. 2010 Nov;76(21):7328-31. doi: 10.1128/AEM.01606-10. Epub 2010 Sep 17.

Novel activity of Rhodobacter sphaeroides spheroidene monooxygenase CrtA expressed in Escherichia coli.

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1
Department of Molecular Science and Technology, Ajou University, Woncheon-dong, Yeongtong-gu, Suwon 443-749, South Korea. pclee@ajou.ac.kr

Abstract

The spheroidene monooxygenase CrtA of Rhodobacter sphaeroides introduces a keto group and/or hydroxy group at the ends of nonnative substrates in Escherichia coli, resulting in the production of novel oxocarotenoids. The heme-containing CrtA is not a P450 enzyme but a new type of oxygenase.

PMID:
20851979
PMCID:
PMC2976243
DOI:
10.1128/AEM.01606-10
[Indexed for MEDLINE]
Free PMC Article
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