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Mol Imaging Biol. 2011 Oct;13(5):995-1002. doi: 10.1007/s11307-010-0412-z.

Preclinical imaging of therapy response using metabolic and apoptosis molecular imaging.

Author information

1
Department of Nuclear Medicine, University Hospital Gasthuisberg Leuven, Leuven, Belgium.

Abstract

PURPOSE:

Early after therapy, 2-deoxy-2-[(18)F]fluoro-D-glucose ([(18)F]FDG) imaging is not always reliable due to the influx of inflammatory cells while apoptosis imaging offers a direct and early measurement of therapy effects. This study uses an improved apoptosis probe ((99m)Tc-hAnxA5) in combination with [(18)F]FDG imaging to evaluate therapy response.

PROCEDURES:

Daudi tumor tissue was implanted in the spleen of SCID mice. Treatment was performed with adriamycin and cyclophosphamide. Sequential [(18)F]FDG-positron emission tomography (PET) was acquired over 6 days and (99m)Tc-hAnxA5-SPECT was performed before and 1 day after therapy.

RESULTS:

On day 1, therapy induced apoptosis was visualized with (99m)Tc-hAnxA5 without a measurable change in [(18)F]FDG uptake. [(18)F]FDG uptake decreased significantly on day 3 and was even more pronounced on day 6.

CONCLUSION:

In this preclinical model, (99m)Tc-hAnxA5 imaging was able to detect apoptosis before metabolic changes were measured. These results confirm the value of apoptosis imaging for therapy response and give more insight in [(18)F]FDG imaging and its parameters to evaluate response.

PMID:
20848227
DOI:
10.1007/s11307-010-0412-z
[Indexed for MEDLINE]
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