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Int J Pediatr Otorhinolaryngol. 2010 Aug;74(8):864-8. doi: 10.1016/j.ijporl.2010.04.014.

Mucin gene expression and mouse middle ear epithelium.

Author information

1
Division of Pediatric Otolaryngology, Medical College of Wisconsin, Children's Hospital of Wisconsin, 9000 W Wisconsin Avenue, Milwaukee, WI 53226, United States. kersch@mcw.edu

Abstract

OBJECTIVES:

To investigate the expression of recently identified human mucin genes in an in vitro model of cultured mouse middle ear epithelial cells (MMEEC).

METHODS:

MMEEC were established, RNA was extracted and primers were designed for RT-PCR to assess for expression of mucin genes Muc1, Muc2, Muc3, Muc4, Muc5AC, Muc5B, Muc6, Muc7, Muc8, Muc9, Muc10, Muc11/12, Muc13, Muc15, Muc16, Muc17, Muc18, Muc19 and Muc20 expression.

RESULTS:

Mucin genes Muc1, Muc2, Muc3, Muc4, Muc5AC, Muc5B, Muc9, Muc10, Muc13, Muc15, Muc16, Muc18, Muc19 and Muc20 were identified and expressed in MMEEC. The genes Muc6, Muc7, Muc8, Muc11/12 and Muc17 were not identified.

CONCLUSION:

Many of the mucin genes that have been recently identified in human MEE and chinchilla MEE are also expressed in MMEEC. There are differences in expression, however, which may have implications in utilizing various animal models for study of middle ear physiology and pathogenesis; specifically as it relates to mucin gene expression.

PMID:
20846498
PMCID:
PMC3327946
DOI:
10.1016/j.ijporl.2010.04.014
[Indexed for MEDLINE]
Free PMC Article

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