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J Cereb Blood Flow Metab. 2010 Nov;30(11):1821-4. doi: 10.1038/jcbfm.2010.160. Epub 2010 Sep 15.

Determination of the brain-blood partition coefficient for water in mice using MRI.

Author information

1
Department of Experimental Neurology, Charité-Universitätsmedizin, Center for Stroke Research Berlin, Berlin, Germany. christoph.leithner@charite.de

Abstract

Cerebral blood flow (CBF) quantification is a valuable tool in stroke research. Mice are of special interest because of the potential of genetic engineering. Magnetic resonance imaging (MRI) provides repetitive, noninvasive CBF quantification. Many MRI techniques require the knowledge of the brain-blood partition coefficient (BBPC) for water. Adopting an MRI protocol described by Roberts et al (1996) in humans, we determined the BBPC for water in 129S6/SvEv mice from proton density measurements of brain and blood, calibrated with deuterium oxide/water phantoms. The average BBPC for water was 0.89 ± 0.03 mL/g, with little regional variation within the mouse brain.

PMID:
20842161
PMCID:
PMC3023928
DOI:
10.1038/jcbfm.2010.160
[Indexed for MEDLINE]
Free PMC Article

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