Isolation and identification of two steroid compounds from Oviductus Ranae

Nat Prod Res. 2010 Oct;24(16):1518-22. doi: 10.1080/14786419.2010.484391.

Abstract

In this study, cholest-5-en-3β,7β-diol and 3β-hydroxy-cholest-5-en-7-one were isolated from Oviductus Ranae by column chromatographies on Sephadex LH-20, octadecylsilyl (ODS) and pre high-performance liquid chromatography (HPLC). Their structures were elucidated on the basis of chemical and spectral analyses, including electrospray ionisation-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR). The above two compounds were obtained from Oviductus Ranae for the first time.

MeSH terms

  • Animals
  • Cholestenones / chemistry
  • Cholestenones / isolation & purification*
  • Female
  • Hydroxycholesterols / chemistry
  • Hydroxycholesterols / isolation & purification*
  • Materia Medica / chemistry*
  • Medicine, Chinese Traditional
  • Molecular Structure
  • Rana temporaria
  • Steroids / chemistry
  • Steroids / isolation & purification*

Substances

  • 3beta-hydroxy-cholest-5-en-7-one
  • Cholestenones
  • Hydroxycholesterols
  • Materia Medica
  • Oviductus Ranae
  • Steroids
  • cholest-5-en-3 beta,7 alpha-diol