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Methods Mol Biol. 2010;673:189-202. doi: 10.1007/978-1-60761-842-3_11.

Mass spectrometric protein identification using the global proteome machine.

Author information

1
The Rockefeller University, New York, NY, USA. fenyo@rockefeller.edu

Abstract

Protein identification by mass spectrometry is widely used in biological research. Here, we describe how the global proteome machine (GPM) can be used for protein identification and for validation of the results. We cover identification by searching protein sequence collections and spectral libraries as well as validation of the results using expectation values, rho-diagrams, and spectrum databases.

PMID:
20835799
PMCID:
PMC3757509
DOI:
10.1007/978-1-60761-842-3_11
[Indexed for MEDLINE]
Free PMC Article

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