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Curr Opin Neurobiol. 2010 Oct;20(5):667-75. doi: 10.1016/j.conb.2010.08.002. Epub 2010 Sep 15.

Semi-automated reconstruction of neural circuits using electron microscopy.

Author information

1
Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA. mitya@janelia.hhmi.org

Abstract

Reconstructing neuronal circuits at the level of synapses is a central problem in neuroscience, and the focus of the nascent field of connectomics. Previously used to reconstruct the C. elegans wiring diagram, serial-section transmission electron microscopy (ssTEM) is a proven technique for the task. However, to reconstruct more complex circuits, ssTEM will require the automation of image processing. We review progress in the processing of electron microscopy images and, in particular, a semi-automated reconstruction pipeline deployed at Janelia Farm. Drosophila circuits underlying identified behaviors are being reconstructed in the pipeline with the goal of generating a complete Drosophila connectome.

PMID:
20833533
DOI:
10.1016/j.conb.2010.08.002
[Indexed for MEDLINE]

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