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Nano Lett. 2010 Oct 13;10(10):4249-52. doi: 10.1021/nl1027638.

Far-field autofluorescence nanoscopy.

Author information

1
Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gttingen, Germany.

Abstract

We demonstrate far-field optical imaging at the nanoscale with unlabeled samples. Subdiffraction resolution images of autofluorescent samples are obtained by depleting the ground state of natural fluorophores by transferring them to a metastable dark state and simultaneously localizing those fluorophores that are transiently returning. Our approach is based on the insight that nanoscopy methods relying on stochastic single-molecule switching require only a single fluorescence on-off cycle to yield an image, a condition fulfilled by various biomolecules. The method is exemplified by recording label-free nanoscopy images of thylakoid membranes of spinach chloroplasts.

PMID:
20831171
DOI:
10.1021/nl1027638
[Indexed for MEDLINE]

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